How to invert and remove this part?

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

I am trying to perform an image analysis. I made the stroke selection and selected the required area. After this I made invert selection of the region outside the selection. But I am clueless of how I remove the inner region (area inside the stroke selection).

I appreciate any help from you guys.

Best
Highlander

Wallace · **Posted:** Tue Jan 09, 2018 11:31 pm **(#2)**

I first duplicated the layer and ran,
Color> Desaturate> Louminosicty.

The I applied a Color Curve.

Using the Fuzzy Select Tool.
I selected the inner portion of this image.

Then switched back to the bottom layer,
with the selection still active and cleared the pixels from the inner portion of the image.

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

Dear Wallace,

Greetings from Japan. Thank you very much for the help!! This method definitely works for my analysis. I have another question. Is it possible to remove the stroke selection (the lines I mean). Since I am trying to perform image analysis based on the color of the stain, my next step would be to remove the lines and then perform image analysis using ImageJ software.

Thank you again for the kind help.

Highlander

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

Wallace wrote:

I first duplicated the layer and ran,
Color> Desaturate> Louminosicty.
[ Image ]

The I applied a Color Curve.
[ Image ]

Using the Fuzzy Select Tool.
I selected the inner portion of this image.
[ Image ]

Then switched back to the bottom layer,
with the selection still active and cleared the pixels from the inner portion of the image.
[ Image ]

Dear Wallace,

Greetings from Japan. Thank you very much for the help!! This method definitely works for my analysis. I have another question. Is it possible to remove the stroke selection (the lines I mean). Since I am trying to perform image analysis based on the color of the stain, my next step would be to remove the lines and then perform image analysis using ImageJ software.

Thank you again for the kind help.

Highlander

Wallace · **Posted:** Wed Jan 10, 2018 1:37 am **(#5)**

Highlander wrote:

Wallace wrote:

I first duplicated the layer and ran,
Color> Desaturate> Louminosicty.
[ Image ]

The I applied a Color Curve.
[ Image ]

Using the Fuzzy Select Tool.
I selected the inner portion of this image.
[ Image ]

Then switched back to the bottom layer,
with the selection still active and cleared the pixels from the inner portion of the image.
[ Image ]

Dear Wallace,

Greetings from Japan. Thank you very much for the help!! This method definitely works for my analysis. I have another question. Is it possible to remove the stroke selection (the lines I mean). Since I am trying to perform image analysis based on the color of the stain, my next step would be to remove the lines and then perform image analysis using ImageJ software.

Thank you again for the kind help.

Highlander

Perhaps this could be done, but due to it's size.
It would be difficult to accomplish using the image you provided.

I might be wrong and if so,
I'm sure someone may come forward and show us how this might be done.

sallyanne · **Posted:** Wed Jan 10, 2018 8:56 am **(#6)**

I dont use them, (just had a quick look though). Instead of stroking a selection - why not save it to a channel? You can still see your selection without having the stroke there.

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

Wallace wrote:

Highlander wrote:

Wallace wrote:

I first duplicated the layer and ran,
Color> Desaturate> Louminosicty.
[ Image ]

The I applied a Color Curve.
[ Image ]

Using the Fuzzy Select Tool.
I selected the inner portion of this image.
[ Image ]

Then switched back to the bottom layer,
with the selection still active and cleared the pixels from the inner portion of the image.
[ Image ]

Dear Wallace,

Greetings from Japan. Thank you very much for the help!! This method definitely works for my analysis. I have another question. Is it possible to remove the stroke selection (the lines I mean). Since I am trying to perform image analysis based on the color of the stain, my next step would be to remove the lines and then perform image analysis using ImageJ software.

Thank you again for the kind help.

Highlander

Perhaps this could be done, but due to it's size.
It would be difficult to accomplish using the image you provided.

I might be wrong and if so,
I'm sure someone may come forward and show us how this might be done.

Thanks Wallace. I tried to do the steps that you did, but was not able to do the last step (switching back to the bottom layer, with the selection still active and cleared the pixels from the inner portion of the image).

I am sorry I am pretty new to image editing and just know a thing or two. How do I switch back to the layer and clear the pixels.

Regards,

Highlander

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

sallyanne wrote:

I dont use them, (just had a quick look though). Instead of stroking a selection - why not save it to a channel? You can still see your selection without having the stroke there.

Hi Sallyanne, thank you for the suggestion. I have no clue how to do that, since I am pretty new to GIMP. I would highly appreciate if you could help me how I can do that.

Regards,

Highlander

Wallace · **Posted:** Wed Jan 10, 2018 10:25 am **(#9)**

Highlander wrote:

Thanks Wallace. I tried to do the steps that you did, but was not able to do the last step (switching back to the bottom layer, with the selection still active and cleared the pixels from the inner portion of the image).

I am sorry I am pretty new to image editing and just know a thing or two. How do I switch back to the layer and clear the pixels.

Regards,

Highlander

Turn the visibility of the top layer off.
By left-clicking on the tiny eye to the left of it's layer.
This will make the bottom layer visible in the larger main canvas.

Make the bottom layer the active visible layer.
By left-clicking on this layer,
within the layers dialog.
With the bottom layer now the active visible layer.
You can clear the inner portion of this layer.
Using the selection that was made from the top layer.

sallyanne · **Posted:** Wed Jan 10, 2018 11:41 am **(#10)**

Make your selection over your image. Then go to selection (up in the top menu), go down to 'save to channel'.
Then continue on as Wallace has advised then by turning the channel eye on and off you can see where your selection was (it is turned into a selection mask).
As I said I dont use channels myself and maybe someone who knows them can explain better.
I had a look what could be done because of your question

and I think it would save you stroking your selection esp if you want to get rid of it later.

I'm off to bed now..late here...erm early morning, I hope you get your problem gets solved.

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

Wallace wrote:

Highlander wrote:

Thanks Wallace. I tried to do the steps that you did, but was not able to do the last step (switching back to the bottom layer, with the selection still active and cleared the pixels from the inner portion of the image).

I am sorry I am pretty new to image editing and just know a thing or two. How do I switch back to the layer and clear the pixels.

Regards,

Highlander

Turn the visibility of the top layer off.
By left-clicking on the tiny eye to the left of it's layer.
This will make the bottom layer visible in the larger main canvas.
[ Image ]

Make the bottom layer the active visible layer.
By left-clicking on this layer,
within the layers dialog.
With the bottom layer now the active visible layer.
You can clear the inner portion of this layer.
Using the selection that was made from the top layer.
[ Image ]

Hi there, sorry for the late reply. I really appreciate your help!! Thank you for helping me out.

Best regards,

Highlander

Highlander · **Joined:** Jan 09, 2018 **Posts:** 17

sallyanne wrote:

Make your selection over your image. Then go to selection (up in the top menu), go down to 'save to channel'.
Then continue on as Wallace has advised then by turning the channel eye on and off you can see where your selection was (it is turned into a selection mask).
As I said I dont use channels myself and maybe someone who knows them can explain better.
I had a look what could be done because of your question

and I think it would save you stroking your selection esp if you want to get rid of it later.

I'm off to bed now..late here...erm early morning, I hope you get your problem gets solved.

Hi Sallyanne, sorry for the late response, I really appreciate your help. I will try using the method you have suggested.

Best regards,

Highlander